Long-Term Culture of Giardia lamblia in Cell Culture Medium Requires Intimate Association with Viable Mammalian Cells
نویسندگان
چکیده
منابع مشابه
Modeling Long-Term Host Cell-Giardia lamblia Interactions in an In Vitro Co-Culture System
Globally, there are greater than 700,000 deaths per year associated with diarrheal disease. The flagellated intestinal parasite, Giardia lamblia, is one of the most common intestinal pathogens in both humans and animals throughout the world. While attached to the gastrointestinal epithelium, Giardia induces epithelial cell apoptosis, disrupts tight junctions, and increases intestinal permeabili...
متن کاملAXENIC CULTURE AND CRYOPRESERVATION OF GIARDIA LAMBLIA ISOLATED IN IRAN
Giardia lamblia cysts were isolated from fecal samples of 55 symptomatic and asymptomatic patients with giardiasis. The cysts were harvested and purified using the sucrose gradient method. IS The excystation of G. lamblia was obtained by treating them with HCI (0.01N)', then they were transferred on to modified TYI-S-33 medium. Out of 55 stool samples 17 cases were excysted with a rate of ...
متن کاملIsolation and long-term culture of diploid mammalian cell lines.
Cells have been established in continuous culture from human and animal tissues, both normal and carcinomatous. From 197 specimens, 105 could be maintained in vitro for varying lengths of time, 27 for more than 6 months. In all cases the cells were found to be diploid. Cell lines with both epithelial and fibroblastic morphology have been isolated in medium 199 with added fetal calf serum, but a...
متن کاملModified PEHPS Medium as an Alternative for the In Vitro Culture of Giardia lamblia
Commercial culture media present interlot variations in biological activity. We have previously designed a homemade and economic culture medium, PEHPS medium, for the axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. Trophozoites of amoebae and trichomonads grow well in this medium. Furthermore, the medium is stable for several months when stored frozen or refrigerated. The...
متن کاملaxenic culture and cryopreservation of giardia lamblia isolated in iran
giardia lamblia cysts were isolated from fecal samples of 55 symptomatic and asymptomatic patients with giardiasis. the cysts were harvested and purified using the sucrose gradient method. is the excystation of g. lamblia was obtained by treating them with hci (0.01n)', then they were transferred on to modified tyi-s-33 medium. out of 55 stool samples 17 cases were excysted with a rate of ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2019
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.00639-19